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了解中脑神经前体细胞和纹状体神经前体细胞在Mes4 2细胞及细胞因子作用下的体外诱导分化情况。采用含细胞因子或 /和Mes4 2细胞条件培养基和细胞膜碎片的分化培养基培养中脑神经前体细胞和纹状体神经前体细胞 ,孵育 7d后行免疫细胞化学染色了解其体外诱导分化情况。IL 1α(10 0pg/ml) ,IL 11(1ng/ml) ,GDNF(1μg/ml)和LIF(1ng/ml)可诱导中脑神经前体细胞分化成TH阳性神经元 ,增加分化的TH阳性细胞数目。Mes4 2细胞条件培养基和其细胞膜裂解碎片以及细胞因子IL 11,LIF ,GDNF ,IL 1α共同培养神经前体细胞 ,诱导分化的TH阳性细胞数目无明显增加 ,但TH阳性细胞的形态更趋成熟。而纹状体神经前体细胞在上述分化培养基内鲜见TH阳性细胞。中脑神经前体细胞可在体外被诱导分化成TH阳性神经元 ,可能成为帕金森病移植治疗的供体细胞
To understand the neural stem cells and striatal neural precursor cells in Mes4 2 cells and cytokines in vitro induced differentiation. Cultured neural precursor cells and striatum neural precursor cells were cultured in differentiation medium containing cytokines and / or Mes4 2 cell conditioned media and cell membrane fragments. Immunocytochemical staining was used to detect the differentiation of neural precursor cells and striatum in vitro . IL 1α (10 μg / ml), IL 11 (1 ng / ml), GDNF (1 μg / ml) and LIF (1 ng / ml) induce mesencephalic neural progenitor cells to differentiate into TH positive neurons, The number of cells. Mes4 2 cells conditioned medium and its cell membrane fragmentation and cytokines IL 11, LIF, GDNF, IL 1α co-cultured neural precursor cells, the number of induced TH positive cells did not increase significantly, but the morphology of TH positive cells more mature . The striatal neural progenitor cells in the above differentiation medium rare TH positive cells. Midbrain progenitor cells can be induced to differentiate into TH-positive neurons in vitro and may become donor cells for Parkinson’s disease transplant