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利用蛋白纤维平板法对1株蛹虫草(Cordyceps militaris)菌株液体发酵产生纤溶酶的碳氮条件进行系统优化;通过单因素试验筛选最佳碳源和氮源,再根据单因素试验结果进行正交设计实验优化该菌株产纤溶酶的最佳配方。结果表明:产纤溶酶的最佳碳源为乳糖,纤溶酶活性达118.69U/mL,其次是不加碳源的空白对照,活性为109.12U/mL;蚕蛹粉是该菌株产纤溶酶的最佳氮源,活性可达118.69U/mL,其次是酵母浸膏,活性为29.10U/mL;纤溶酶产量与菌丝体生物量之间无必然相关性;产纤溶酶的最佳培养基为乳糖10g/L、蚕蛹粉10g/L、酵母浸膏5g/L,获得的纤溶酶活性高达142.26U/mL。
The carbon and nitrogen conditions of liquid fibrinolytic enzyme production in a Cordyceps militaris strain were systematically optimized by protein fiber plate method. The best carbon and nitrogen sources were screened by single factor test and then positive Design experiments to optimize the strain producing plasmin the best formula. The results showed that the optimal carbon source for producing plasmin was lactose, the activity of plasmin was 118.69U / mL, followed by the blank control without carbon source, the activity was 109.12U / mL. The silkworm pupa powder was the fibrinolytic The optimum nitrogen source of the enzyme activity up to 118.69U / mL, followed by the yeast extract, the activity of 29.10U / mL; fibrinolytic enzyme production and mycelial biomass without a correlation between the; plasminogen The best culture medium was lactose 10g / L, silkworm pupa powder 10g / L, yeast extract 5g / L, and the obtained plasmin activity was as high as 142.26U / mL.