论文部分内容阅读
目的:观察机械性透室壁心肌血管重建术（Transmyocardial revascularization,TMR）管道内转入VEGF基因后血管再生与重建的形态学效果,并探索TMR结合转基因技术的新方法。方法:利用高速空心钻头在家兔左室前壁进行TMR,形成透室壁性心肌管道。实验组的心肌管道内注入编码VEGFcDNA质粒;对照组仅做单纯机械性TMR。动物于术后不同时间处死,利用组织切片染色法,对照观察两组TMR管道内血管再生与重建状况。结果:实验组管道内新生小血管的密度月数量较高,与对照组相比具有非常显著性差异（P<0.001）。结论:机械TMR管道内注入VEGcDNA质粒后能有效地促进其血管生成,机械TMR结合转基因技术促血管生成作用明显优于单纯机械性TMR。
OBJECTIVE: To observe the morphological effects of vascular revascularization and remodeling after transmyocardial revascularization (TMR) transsection into the vascular endothelial cells and to explore a new method of TMR binding with transgenic technology. Methods: TMR was performed on the anterior wall of rabbit left ventricle by using a high speed hollow drill to form a transmural myocardial duct. In the experimental group, the VEGFcDNA plasmid was injected into the myocardium of the experimental group, while the control group was only a simple mechanical TMR. The animals were sacrificed at different times after operation. Tissue section staining was used to observe the status of vascular regeneration and reconstruction in both groups. Results: The number of newborn blood vessels in experimental group was higher than that in control group (P <0.001). CONCLUSION: The injection of VEGc DNA plasmid into mechanical TMR can effectively promote its angiogenesis. The mechanical angiogenesis effect of mechanical TMR combined with transgenic technology is obviously better than pure mechanical TMR.