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目的 探讨脑脊液和血清S 10 0b蛋白含量对Guillain Barre综合征 (GBS)患者施万细胞损伤的评估价值。方法 采用酶联免疫吸附试验双抗体夹心法对 5 0例GBS患者 (重型组 19例 ,轻型组 31例 )和 2 2名正常人的脑脊液和血清S 10 0b蛋白含量进行检测 ,同时行脑脊液细胞学检查。对GBS患者还进行了脑脊液和血清的动态检测。结果 (1)两组GBS患者脑脊液S 10 0b蛋白含量均高于对照组 (均P <0 0 1) ,重型组与轻型组差异亦有显著意义 (P <0 0 1) ;血清S 10 0b蛋白含量重型组患者高于轻型组和对照组 (均P <0 0 5 ) ,轻型组与对照组差异无显著意义 (P >0 0 5 )。 (2 )重型组患者脑脊液单核细胞比例明显高于轻型组和对照组 (均P <0 0 1) ,轻型组与对照组间差异无显著意义(P >0 0 5 )。 (3)GBS患者脑脊液S 10 0b蛋白含量随脑脊液单核细胞比例增减及病情的轻重而发生相应的变化。结论 脑脊液和血清S 10 0b蛋白含量与GBS患者病情严重程度相关。
Objective To investigate the value of cerebrospinal fluid (CSF) and serum S 10 0b protein in the evaluation of Schwann cell injury in patients with Guillain-Barre syndrome (GBS). Methods Serum and serum S 10 0b protein levels in 50 patients with GBS (19 in the severe group and 31 in the light group) and 22 normal controls were detected by enzyme-linked immunosorbent assay (ELISA) with double antibody sandwich method. Cerebrospinal fluid School inspection. GBS patients also conducted a dynamic detection of cerebrospinal fluid and serum. Results (1) The content of S 10 0b protein in cerebrospinal fluid of two groups of GBS patients was significantly higher than that of the control group (all P <0.01), and there was significant difference between the severe group and the light group (P <0.01) The patients with heavy protein content in the light group were higher than those in the light group and the control group (P <0.05). There was no significant difference between the light group and the control group (P> 0.05). (2) The proportion of cerebrospinal fluid mononuclear cells in severe group was significantly higher than that in light group and control group (all P <0.01). There was no significant difference between light group and control group (P> 0.05). (3) The content of S 10 0b protein in cerebrospinal fluid of patients with GBS changes correspondingly with the increase or decrease of the proportion of mononuclear cells in cerebrospinal fluid and the severity of disease. Conclusion The contents of S 10 0b protein in cerebrospinal fluid and serum are related to the severity of GBS patients.