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目的 探讨基质金属蛋白酶 2 (matrixmetalloproteinase2 ,MMP 2 )的表达与侵袭性垂体腺瘤相关性研究 .以寻找垂体腺瘤侵袭性可能的发病机制 .方法 用免疫组化的方法对 54例垂体瘤患者的组织标本进行研究 .并对其中 1 6例患者采用逆转录 聚合酶链反应 (RT PCR)的方法对MMP 2mRNA的表达进行研究 .免疫组化结果用半定量的方法进行分析 .结果 54例垂体瘤患者中 ,1 2例为侵袭性垂体腺瘤 ,4 2例为非侵袭性垂体腺瘤 .免疫组化显示 ,侵袭性垂体腺瘤的MMP 2的表达明显高于非侵袭性垂体腺瘤 (分别为 3 9± 1 6 ,2 3± 1 4 ,P <0 0 0 1 ) .大型垂体腺瘤和微垂体腺瘤 ,以及功能性和非功能性垂体腺瘤的MMP 2的表达则无明显差别 .MMP 2的表达与Ki 6 7的表达无明显相关性 (r=- 0 0 5;P >0 0 5) .侵袭性垂体腺瘤MMP 2mRNA的表达 ,明显高于非侵袭性垂体腺瘤 (P <0 0 5) .结论 MMP 2的高表达与肿瘤的侵袭性密切相关 ,但与肿瘤的大小及分泌功能无明显关系 .MMP 2可以作为肿瘤侵袭性一项有效的指标 .
Objective To investigate the correlation between the expression of matrix metalloproteinase 2 (MMP 2) and invasive pituitary adenomas in order to find out the possible pathogenesis of invasive pituitary adenomas.Methods Immunohistochemical methods were used to detect the expression of matrix metalloproteinase 2 Tissue specimens were studied, and the expression of MMP 2 mRNA was studied by reverse transcription polymerase chain reaction (RT-PCR) in 16 of them.Immunohistochemistry results were analyzed by semi-quantitative methods.Results 54 cases of pituitary tumor Among the patients, 12 were invasive pituitary adenomas and 42 were non-invasive pituitary adenomas.Immunohistochemistry showed that the expression of MMP-2 in invasive pituitary adenomas was significantly higher than that in non-invasive pituitary adenomas 3 9 ± 1 6, 2 3 ± 1 4, P 0 01). There was no significant difference in the expression of MMP 2 between large and small pituitary adenomas and functional and non-functional pituitary adenomas The expression of MMP 2 was not significantly correlated with the expression of Ki 6 7 (r = - 0 05; P 0 05) .The expression of MMP 2 mRNA in invasive pituitary adenomas was significantly higher than that in noninvasive pituitary adenomas P <0 05) .Conclusion The high expression of MMP 2 and tumor invasion It is closely related to aggressiveness, but not to tumor size and secretory function.MMP 2 can be used as a valid indicator of tumor invasiveness.