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14例正常人外周血经半微量全血培养后作了Giemsa 和荧光色素(吖啶橙AO)染色,并进行定量分析比较。结果表明,荧光微核检出率显著高于Giemsa(P<0.01)。据此,笔者建立了半微量血培养荧光微核分析法。本法与国外类似方法相比有如下优点:①将检出率最高的半微量血培养法与荧光显示微核法结合在一起,敏感性、可靠性和可见度显著提高:②仅用PBS 封片,既简便,又可随时复染,色差仍很明显。③用BG3激发滤片和Y 475屏障滤片代替BG—12和0-515或0-530,背景为天蓝色,使色彩鲜艳并增加衬度。这种方法的建立对于评定药物的遗传毒理和优生学产前诊断等均有一定意义。
Fourteen normal human peripheral blood was stained with Giemsa and fluorescent dye (acridine orange AO) after semi-micro whole blood culture, and compared quantitatively. The results showed that the detection rate of fluorescence micronucleus was significantly higher than Giemsa (P <0.01). Accordingly, I established a semi-micro-blood culture fluorescence micronucleus analysis. This method compared with similar foreign methods have the following advantages: ① the highest detection rate of semi-micro-blood culture method and fluorescence display micronuclei together, the sensitivity, reliability and visibility significantly increased: , Not only easy, but also at any time countersunk, color is still very obvious. ③ BG3 excitation filter and Y475 barrier filter instead of BG-12 and 0-515 or 0-530, the background is sky blue, so colorful and increase the contrast. The establishment of this method is of certain significance for assessing the genetic toxicology of drugs and prenatal diagnosis of eugenics.