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三氟羧草醚对雄、雌小白鼠急性经口ＬＤ５０分别为１４７０ｍｇ／ｋｇ和２３３０ｍｇ／ｋｇ；对雄、雌大白鼠急性经口ＬＤ５０分别为１２６０ｍｇ／ｋｇ和１７１０ｍｇ／ｋｇ，急性经皮ＬＤ５０＞４６４０ｍｇ／ｋｇ；微核和精子畸形试验结果为阴性。
Acifluorfen on male and female mice acute oral LD50 were 1470mg / kg and 2330mg / kg; male and female rats acute oral LD50 were 1260mg / kg and 1710mg / kg, acute percutaneous LD50> 4640mg / kg; micronucleus and sperm abnormality test results were negative.