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目的通过去卵巢大鼠的模型,观察雌激素缺乏后对学习和记忆的影响以及突触素表达的情况。方法实验选用成年Wistar大鼠33只,随机分为对照组、去卵巢组、补充雌激素组(去卵巢),每组11只,实验周期为4个月,观察指标:血中的雌二醇含量、记忆跳台潜伏期和错误次数以及海马结构中突触素表达情况。结果①血清中雌二醇浓度:对照组为(46.7±25.3)pg/mL;去卵巢组为(0.3±0.3)pg/mL;补充雌激素组为(144.4±78.9)pg/mL,各组间比较差异均有统计学意义(P<0.05)。②跳台记忆潜伏期和错误次数:对照组分别为(148.7±19.6)s、(2.9±1.1)次;去卵巢组为(85.6±16.8)s、(7.9±2.9)次;补充雌激素组为(101.6±18.6)s、(4.6±1.1)次;除了去卵巢组的记忆潜伏期与补充雌激素组比较无显著差异外(P>0.05),其他各组之间差异均有统计学意义(P<0.05)。③海马结构突触素的表达:去卵巢组海马的神经元胞浆内有少许棕黄色的颗粒,呈弱阳性;补充雌激素组与对照组相似,呈强阳性表达。结论雌激素缺乏可以导致大鼠跳台试验的错误次数增加和记忆潜伏期延长,突触素表达减少;补充雌激素有所好转,同时突触素表达增多,推测大鼠雌激素缺乏后的认知功能减退可能与海马结构中突触素表达减少有关。
Objective To observe the effect of estrogen deficiency on learning and memory and synaptophysin expression in ovariectomized rats. Methods Thirty-three adult Wistar rats were randomly divided into control group, ovariectomized group and estrogen-supplemented group (ovariectomized group), with 11 rats in each group. The experimental period was 4 months. The observation indexes included estradiol Content, latency and error of memory jumping platform and synaptophysin expression in hippocampal formation. Results ① Serum estradiol concentration was (46.7 ± 25.3) pg / mL in the control group, (0.3 ± 0.3) pg / mL in the ovariectomized group and (144.4 ± 78.9) pg / mL in the estrogen replacement group There was significant difference between the two groups (P <0.05). (2) The latency and the number of errors in jumping memory were (148.7 ± 19.6) s and (2.9 ± 1.1) times in the control group, (85.6 ± 16.8) s and (7.9 ± 2.9) times in the ovariectomized group, respectively 101.6 ± 18.6) s, (4.6 ± 1.1) times, respectively. Except for the memory latency of ovariectomized group was not significantly different from that of estrogen supplementation group (P> 0.05), the differences among the other groups were statistically significant (P < 0.05). (3) The expression of synaptophysin in hippocampal formation: Some brownish-yellow granules in the cytoplasm of hippocampus of ovariectomized group were weakly positive; the estrogen-supplemented group was similar to the control group and showed strong positive expression. Conclusions Estrogen deficiency can lead to an increase in the number of errors and latency of memory test in rats, and reduce the expression of synaptophysin. Supplementation of estrogen improved and the expression of synaptophysin increased, suggesting that cognitive function after estrogen deficiency in rats The decrease may be related to the decreased expression of synaptophysin in the hippocampus.