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利用生物信息学预测rVvhA的141-335位氨基酸片段有膜成孔模序。基因克隆表达得到95%以上纯度的rMpf,电子透射电镜观察其能够抑制Hela细胞生长且呈剂量依赖性,即0.8,1.6,2.4μg/mL rMpf作用8 h后,细胞和线粒体形态均发生凋亡和坏死改变,细胞内活性氧产生明显,线粒体膜电位下降,mPTP荧光检测膜通道孔活性增强。以上结果表明,rMpf具有诱导Hela细胞损伤的生物学活性,可通过改变膜通透性引起细胞凋亡。
Bioinformatics prediction of the 141-335 amino acid fragment of rVvhA membrane pore mode. The results showed that rMpf was more than 95% purity by electron transmission electron microscopy, which could inhibit the growth of Hela cells in a dose-dependent manner by electron microscopy. After treated with 0.8, 1.6 and 2.4 μg / mL rMpf for 8 h, the morphology of cells and mitochondria were both apoptotic And necrosis, intracellular reactive oxygen species were significantly decreased, mitochondrial membrane potential decreased, mPTP fluorescence detection membrane channel activity increased. The above results show that, rMpf has the biological activity of inducing Hela cell injury, and can induce cell apoptosis by changing membrane permeability.