以HPV-16DNA重组质粒作探针,用点杂交和Southern吸印杂交方法对临床宫颈癌和宫颈炎活检样品进行了检测,阳性率分别为73.9％(17/23)、53.8％(7/13);并显示HPV-16基因是以整合状态结合于宿主细胞的基因组中。结果提示:①HPV-16感染与宫颈癌的发生有着密切关系;②HPV感染妇女生殖器在我国较普遍,应引起高度重视。 The HPV-16 DNA recombinant plasmids were used as probes to detect clinical cervical cancer and cervicitis biopsy samples by dot hybridization and Southern blot hybridization. The positive rates were 73.9% (17/23) and 53.8% (7/13) respectively ); And shows that the HPV-16 gene is integrated into the host cell’s genome in an integrated state. The results suggest that: (1) HPV-16 infection is closely related to the occurrence of cervical cancer; (2) HPV genital infection in women is more common in our country, which should be given high priority.