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目的 :了解谷胱甘肽 S-转移酶 ( GST)在癌变过程中的作用及是否对肝癌的早期诊断有价值。方法 :以化学致癌剂 2 -乙酰氨基芴诱发大鼠肝癌 ,检测其癌变过程中组织和血清 GST活性的动态变化。结果 :大鼠摄入 2 -乙酰氨基芴 2周后 ,血清 GST活性比正常对照组升高 ;第 4周时 GST活性比正常对照组明显升高 ( P<0 .0 1) ;到第 6周时 ,GST活性达到最高 ,为正常对照组的 11倍之多 ,以后 GST活性逐步缓慢下降 ,到第 12周 GST活性趋于正常水平 ( P>0 .0 5 )。肝组织中 GST比活性上升幅度较快 ,第 4周时已达最高水平 ,而第 6周时恢复到正常对照组水平 ,随后逐步升高 ,到第 12周 ,与正常对照组比较 ,差异没有显著性 ( P>0 .0 5 )。结论 :GST是癌变早期的标志酶 ,血清 GST活性检测对肝癌的早期诊断有一定价值
Objective: To understand the role of glutathione S-transferase (GST) in carcinogenesis and its value in the early diagnosis of liver cancer. Methods: Rat liver cancer was induced by chemical carcinogen 2-acetylamino hydrazine, and the dynamic changes of GST activity in tissues and serum during carcinogenesis were detected. RESULTS: Serum GST activity was increased in rats after 2 weeks of 2-acetylamino hydrazine ingestion compared with normal control group; GST activity was significantly higher in the 4th week than in the normal control group (P < 0.01); At week, GST activity reached the highest level, which was 11 times higher than that of the normal control group. GST activity gradually decreased gradually afterwards, and GST activity tended to normal level at week 12 (P>0.05). GST activity in liver tissue increased rapidly, reaching the highest level at the 4th week, and returned to the normal control group at the 6th week, and then gradually increased. At the 12th week, there was no difference compared with the normal control group. Significant (P > 0.05). Conclusion : GST is a marker enzyme in the early stages of cancer, serum GST activity assay has a certain value for the early diagnosis of liver cancer.