In vitro PRODUCTION OF HAPLOID PLANTS FROM MULBERRY (Morus) ANTHER CULTURE

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Anthers with pollen during the uninucleate to late uninucteate stages are inoculated into dedifferentiation medium, cultured at 28℃ in the dark for 15 days, and then placed under the illumination of 2000 lux 12 h a day. After 35 days, embryoids arc induced, the induction frequency reaching 13.6%. On greening, the embryoids about 3 mm in diameter are transferred to differentiation medium, and the regeneration percentage of plantlets is 37.21%. Plantlets about 2—3 cm in height are transferred first to a rooting medium for 2—4 days, then to a hormoneless medium. The formation frequency of roots is up to 95%. Chromosome examination shows that all embryoids are haploid, and the haploid plant is very successfully induced from mulberry anther culture. Anthers with pollen during the uninucleate to late uninucteate stages are inoculated into dedifferentiation medium, cultured at 28 ° C in the dark for 15 days, and then placed under the illumination of 2000 lux for 12 ha day. After 35 days, embryoids arc induced, the induction frequency reaching 13.6%. On greening, the embryoids about 3 mm in diameter are transferred to the differentiation medium, and the regeneration percentage of plantlets is 37.21%. Plantlets about 2-3 cm in height are transferred first to a rooting medium for 2-4 days, then to a hormoneless medium. The formation frequency of roots is up to 95%. Chromosome examination shows that all embryoids are haploid, and the haploid plant is very successfully induced from mulberry anther culture.
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