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目的探讨大蒜油对利福平(rifampicin,RIF)和异烟肼(isoniazid,INH)所致大鼠肝损伤的保护作用。方法将60只雄性Wistar大鼠随机分为5组:大蒜油低、中、高剂量处理组、RIF+INH模型组和正常对照组,每组12只。大蒜油低、中、高处理组分别给予相应浓度的大蒜油20、40和80 mg/kg,正常对照组给予等体积的玉米油;2 h后RIF+INH模型组和大蒜油各处理组均给予RIF(75 mg/kg)+INH(75 mg/kg),正常对照组给予等体积的5%淀粉溶液,所有处理每天1次,连续灌胃28 d。观察大鼠体重变化,28 d后测定大鼠血清中丙氨酸转氨酶(alanine transaminases,ALT)、天冬氨酸转氨酶(aspartate transaminases,AST)、总胆红素(total bilirubin,TB)含量、肝重及肝脏系数,同时对肝脏进行病理组织学检查。结果与正常对照组比较,RIF+INH模型组体重无统计学意义,肝重和肝脏系数明显增大(P<0.01),血清中ALT、AST及TB的含量明显升高(P<0.01);与RIF+INH模型组比较,大蒜油低、中和高剂量处理组大鼠体重差异无统计学意义,肝重分别减小3.79%、3.84%和7.73%(P<0.05),脏系数分别减小0.32%、3.25%和3.57%(P<0.05),血清中ALT、AST及TB的含量明显降低(P<0.01或P<0.05)。肝脏病理结果显示,模型组有不同程度细胞浊肿、气球样变,大蒜油处理组没有明显的病理结构改变。结论大蒜油可对利福平和异烟肼联合诱导的肝损伤有保护作用。
Objective To investigate the protective effects of garlic oil on rat liver injury induced by rifampicin (RIF) and isoniazid (INH). Methods Sixty male Wistar rats were randomly divided into 5 groups: low, medium and high dose garlic oil group, RIF + INH model group and normal control group, 12 rats in each group. The garlic oil of low, middle and high treatment groups were given the corresponding concentrations of garlic oil 20,40 and 80 mg / kg, the normal control group was given the same volume of corn oil; 2 h after RIF INH model group and garlic oil treatment group RIF (75 mg / kg) + INH (75 mg / kg) was given to the normal control group. An equal volume of 5% starch solution was given, all treated once daily for 28 days. The body weight of the rats was observed. The content of alanine transaminases (ALT), aspartate transaminases (AST) and total bilirubin (TB) Weight and liver factor, while the liver histopathological examination. Results Compared with the normal control group, the body weight of RIF + INH model group had no statistical significance, the liver weight and liver coefficient increased significantly (P <0.01), the levels of ALT, AST and TB in serum increased significantly (P <0.01). Compared with the RIF + INH model group, there was no significant difference in the body weight of the garlic oil treated with low, medium and high dose of garlic oil, the liver weights decreased by 3.79%, 3.84% and 7.73% (P <0.05) (0.32%, 3.25% and 3.57%, P <0.05). The levels of ALT, AST and TB in serum were significantly decreased (P <0.01 or P <0.05). Liver pathology results showed that the model group had different degrees of cell swelling, balloon-like changes, garlic oil treatment group no obvious pathological changes. Conclusion Garlic oil may have a protective effect on hepatic injury induced by combination of rifampicin and isoniazid.