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1982年夏季,从本校大豆研究室试验田中得到一个顶枯病株,同时伴有皱缩花叶、叶脉坏死、叶柄和茎部坏死条斑、植株矮缩等症状。在防虫温室内分离,得到三个病毒分离物,其中之一是坏死型病毒。在室内对坏死病毒的鉴定结果如下:它的寄主范围广,在接种的9科40种植物中可以侵染34种,人工接种后的寄主反应都是坏死症状。体外抗性强,温度钝化点为90～95℃(10分钟),体外存活期(室温)为80天以上,稀释限点为10~(-7),—10~(-8)。用大
In the summer of 1982, a strain of top blight was obtained from the experimental field of Soybean Research Laboratory of our university. At the same time, there was accompanied with the symptoms of wrinkled mosaic, necrosis of veins, necrosis of petiole and stem, plant dwarfing and other symptoms. Isolated in the pest control greenhouse, three virus isolates were obtained, one of which was a necrotic virus. The identification of necrotic virus in laboratory is as follows: It has a wide range of hosts, 34 species can be inoculated in 40 plants of 9 families inoculated, and host reactions after artificial inoculation are all necrotic symptoms. The in vitro resistance is strong, the temperature passivation point is 90 ~ 95 ℃ (10 minutes), the in vitro survival (room temperature) is more than 80 days, the dilution limit is 10 ~ (-7), -10 ~ (-8). With big