低密度脂蛋白(LDL)在高血糖状态下,其多肽链上赖氨酸残基ε-NH_2易与葡萄糖发生非酶促结合,生成糖基化产物,使LDL功能和代谢异常,被认为在糖尿病患者动脉粥样硬化形成中起重要作用。现已肯定血液中单核细胞移入血管内皮下成熟成巨噬细胞吞噬大量脂质颗粒是动脉粥样硬化斑块中泡沫细胞的主要来源。本实验从健康成人外周血超速离心分离LDL,在无还原剂并接近体内状态下使用80mM葡萄糖与LDL孵育7-10 Low-density lipoprotein (LDL) in the hyperglycemic state, the polypeptide chain lysine residue ε-NH2 easily glucose and non-enzymatic binding, the formation of glycosylation products, so that LDL function and metabolic abnormalities, is considered in the Diabetes patients play an important role in the formation of atherosclerosis. It has been affirmed that blood monocytes migrate into the vascular endothelium and mature into macrophages. The phagocytosis of large numbers of lipid particles is a major source of foam cells in atherosclerotic plaques. In this experiment, LDL was isolated from the supernatant of healthy adult peripheral blood by ultracentrifugation and incubated with LDL for 7-10 in the absence of reducing agent and near-in vivo state using 80 mM glucose