作者报道,将常规应用的尿素酶试验稍经改进,用于1分钟内窥镜室试验检查幽门弯曲菌,可在内窥镜检查完成前作出诊断。作者从40例(男13例、女27例,年龄26～84岁,平均53.3岁)有上消化道症状,拟诊为幽门弯曲菌感染的患者,各采集2份胃窦活检标本。1份标本送微生物实验室,用革兰氏染色和接种于Skirrow培养基,在微氧环境、37℃培养4～7天后检查细菌;并接种于Christensen尿素肉汤中作标准尿素酶试验。另1份标本在内窥镜室内即刻放置于一个内含1ml用去离子水新鲜配制的10%(w/v)、pH6.8尿素液和2滴1%酚红 The authors report that the urease test, which has been routinely applied, has been slightly modified for Campylobacter pylori testing in a 1-minute endoscopy chamber and can be diagnosed before endoscopy is complete. The authors collected two gastric biopsy specimens from 40 patients (13 males and 27 females, aged 26-84 years, mean 53.3 years) with upper gastrointestinal symptoms and suspected patients with Campylobacter pylori infection. One specimen was sent to a microbiology laboratory, stained with Gram stain and seeded in Skirrow medium. Bacteria were examined after incubation for 4 to 7 days in a micro-oxygen environment at 37 ° C and inoculated into Christensen’s urea broth for standard urease tests. Another specimen was immediately placed in an endoscope chamber containing 1 ml of 10% (w / v), pH6.8 urea solution and 2 drops of 1% phenol red, freshly prepared in deionized water