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目的建立肝门部胆管癌神经浸润裸鼠模型。方法将培养的胆管癌细胞系QBC939细胞接种于裸鼠背部皮下,建立裸鼠背部皮下原代胆管细胞癌动物模型,取原代肿瘤组织接种于裸鼠腹腔近肝脏处,建立腹腔二代移植瘤模型,成瘤后行种植瘤组织解剖学和病理学观察。结果肝门部胆管癌背部皮下原代种植瘤成瘤率为100%(5/5),未见神经浸润;腹腔二代移植瘤成瘤率为45%(9/20),神经浸润率为22%(2/9),神经浸润模型成功率为10%(2/20)。病理学观察显示为瘤细胞大小不一,形态多样,不规则;细胞分化程度低,胞浆减少,细胞核大,异形性明显,大小不等,可见核分裂像;无明显腺管样结构。结论肝门部胆管癌细胞具有较强的神经浸润性,应用QBC939细胞系建立肝门部胆管癌神经浸润裸鼠模型为研究肝门部胆管癌神经浸润方式及生物学特性创建了良好的实验平台。
Objective To establish a model of neural invasion in hilar cholangiocarcinoma. Methods The cultured cholangiocarcinoma cell line QBC939 was inoculated subcutaneously in the back of nude mice to establish an animal model of subcutaneous primary cholangiocarcinoma in nude mice. Primary tumor tissues were inoculated into the peritoneal cavity of nude mice near the liver to establish the second generation xenografts of abdominal cavity Anatomical and pathological observation of implanted tumor after tumor formation. Results The rate of tumor formation in the primary subcutaneous subcutaneous excision of hilar cholangiocarcinoma was 100% (5/5), no infiltration of nerve was observed. The rate of tumorigenesis of the second generation of transplanted abdominal tumor was 45% (9/20) 22% (2/9), and the success rate of neural invasion model was 10% (2/20). Pathological observation showed that the tumor cells of different sizes, irregular shape; cell differentiation is low, reduced cytoplasm, large nucleus, heterogeneous obvious, ranging in size, showing mitotic images; no obvious duct-like structure. Conclusions The hilar cholangiocarcinoma cells have strong neural infiltration. The QBC939 cell line was used to establish the model of neural invasion of hilar cholangiocarcinoma in nude mice to establish a good experimental platform for studying the neural infiltration and biological characteristics of hilar cholangiocarcinoma .