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以中国春小麦品种中８６０１、澳大利亚栽培小麦品种Ｓｕｎｓｔａｒ、Ｍｉｌｌｅｗａ作母本，与抗大麦黄矮病毒病（ＢａｒｌｅｙＹｅｌｌｏｗＤｗａｒｆＶｉｒｕｓ，简称ＢＹＤＶ）的小麦－中间偃麦草异附加系Ｌ１杂交，取杂种的幼胚和幼穗作离体培养，获得大量再生植株。经酶联免疫吸附分析（ＥＬＩＳＡ）、限制性内切酶长度多态性分析（ＲＦＬＰ）、染色体数目的检查和田间抗性鉴定，在再生植株回交后代中获得了抗ＢＹＤＶ的杂合易位株，经自交和花药培养纯合，选育出Ｄ１０９１、Ｄ１０９４、Ｄ１６５８、ＴＣ５、ＴＣ６、ＴＣ７等一批抗性稳定或基本稳定的普通小麦选系。以白黑麦６Ｒ二体附加系为抗源，与严重感染白粉病的陕７８５９杂交，经幼胚培养在再生植株回交后代中筛选到白粉病免疫的普通小麦杂合易位系。研究结果表明：在小麦远缘杂交中，组织培养可以作为导入外源基因产生易位的手段之一加以利用。
In this study, hybrids of young wheat and Thinopyrum intermedium L., 8601, Australian cultivated wheat cultivar Sunstar and Millewa, were crossed with L1 of Barley Yellowward Virus (BYDV) Spike for in vitro culture, access to a large number of regenerated plants. By ELISA, restriction fragment length polymorphism analysis (RFLP), chromosome number examination and field resistance identification, the regenerated plant backcrossing progenies obtained BYDV hybrid translocation A series of common wheat lines with stable or basically stable resistance such as D1091, D1094, D1658, TC5, TC6 and TC7 were bred by selfing and anther culture. Crossbred lines of white rye 6R were used as the anti-source to cross with Shaan 7859, which is seriously infected with powdery mildew. The hybrid wheat translocation lines of common wheat immunized with powdery mildew were screened out from the backcrossed generations of regenerated plants by immature embryo culture. The results showed that in wheat distant hybridization, tissue culture can be used as one of the means to introduce foreign genes to produce translocation.