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军团病是医院内肺炎暴发流行的一个重要原因,病原菌多系血清1型嗜肺军团菌(SGl LP)。最近格拉斯哥皇家医院发生一起军团病暴发流行,总共发现16例军团病患者,用快速诊断方法早期有效地控制了该病蔓延。作者采集支气管吸取物和痰标本,用SGl LP 单克隆抗体进行直接荧光抗体试验,同时在缓冲炭酵母提取物的琼脂培基内培养;用间接荧光抗体试验检查血清抗体。符合以下标准之一即可诊断为军团病:①呼吸道分泌物中存在SGl LP;②抗SGlLP 抗体滴度升高4倍;③恢复期SGl LP 抗体滴度≥256。结果16例病人中9例进行了SGl LP 直接荧光
Legionnaires’ disease is one of the major causes of pneumonia outbreaks in hospitals, and the pathogens are mostly serotype 1 Legionella pneumophila (SGL LP). A recent outbreak of Legionnaires ’Disease at the Royal Glasgow Hospital found a total of 16 cases of Legionnaires’ disease and early and effective control of the spread was rapid and diagnostic. The authors collected bronchial aspirates and sputum samples, tested the direct fluorescent antibody with the SGL LP monoclonal antibody and cultured in agar medium with the charcoal-buffered charcoal extract. The serum antibodies were tested by the indirect fluorescent antibody test. In line with one of the following criteria can be diagnosed as Legionnaires’ disease: ① respiratory secretions exist SGl LP; ② anti-SGLLP antibody titer increased 4 times; ③ recovery SGl LP antibody titer ≥ 256. Results Nine of 16 patients underwent SGl LP direct fluorescence