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目的 研究诱导性一氧化氮合酶 ( i NOS)抑制药氨基胍 ( AG)和非选择性 NOS抑制药L- N-硝基精氨甲酯 ( L- NAME)对感染性休克鼠肝肺组织学改变的影响。方法 小鼠腹腔内注射 ( i.p.)内毒素 ( L PS,2 0 mg· kg- 1 )后 4小时随机分为 L PS组 ( n=60 ) ,L PS+L - NAME组 ( 3 0 mg· kg- 1i.p.,n=60 )。和 L PS+AG组 ( 2 0 mg· kg- 1 i.p.,n=60 )。5小时后取受试鼠的肝肺组织进行光镜和电镜检查。并观察 2 4小时内受试鼠的生存率。结果 L PS组、L PS+L- NAME组和 L PS+AG组 2 4小时内生存率分别为 3 7.5 % ,5 %和 64 .3 % ( P<0 .0 5 )。光镜下 L PS组和 L PS+L- NAME组肝细胞和核肿胀 ;肺间质增厚。电镜下可见 L PS组和 L PS+L- NAME组可见肝细胞核、细胞膜和线粒体膜明显破坏 ;肺血气屏障明显增厚。 L PS+AG组肝细胞和肺部的改变明显轻于其它两组。结论 感染性休克中 c NOS介导的一定量的 NO对器官有保护作用。以氨基胍选择性抑制 i NOS合成的 NO,疗效好于非选择性 NOS抑制药
Objective To investigate the effects of inducible nitric oxide synthase (iNOS) inhibitor aminoguanidine (AG) and nonselective NOS inhibitor L-N-nitroammine methyl ester (L-NAME) on liver and lung tissue of septic shock rats The impact of change. Methods Mice were randomly divided into L PS group (n = 60) and LPS + L - NAME group (30 mg · kg -1) 4 hours after intraperitoneal injection of LPS (LPS, 20 mg · kg -1) kg-1i.p., n = 60). And LPS + AG group (20 mg · kg-1 i.p., n = 60). Five hours later, the liver and lung tissues of the test mice were examined by light microscopy and electron microscopy. The survival rate of the test rats within 24 hours was observed. Results The survival rates of LPS group, LPS + L-NAME group and LPS + AG group within 3 hours were 3 7.5%, 5% and 64.3%, respectively (P <0.05). Light microscope, L PS group and L PS + L-NAME group of liver cells and nuclear swelling; interstitial lung thickening. Electron microscopy showed that LPS group and L PS + L-NAME group showed obvious destruction of hepatocyte nucleus, cell membrane and mitochondrial membrane; pulmonary blood gas barrier was significantly thicker. L PS + AG liver cells and lung changes were significantly lighter than the other two groups. Conclusions A certain amount of NO mediated by c NOS in septic shock has a protective effect on organs. With aminoguanidine iNOS synthesis of selective inhibition of NO, better than non-selective NOS inhibitors