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目的研究丹参酮ⅡA、隐丹参酮和丹参酮Ⅰ组合物(组合物)在斑马鱼体内的代谢,探讨斑马鱼用于中药多组分代谢研究的可行性及合理性。方法将斑马鱼暴露于组合物1%DMSO纯净水溶液中,采用高效液相色谱-电喷雾离子阱质谱(HPLC-MS)检测,根据准分子离子峰获得代谢物相对分子质量信息,通过与文献数据或对照品比较,鉴定组合物暴露于斑马鱼24 h后的药液及鱼体中的代谢产物。结果组合物经斑马鱼作用后,产生丹参酮ⅡA和隐丹参酮的羟基化和脱氢产物,未发现丹参酮Ⅰ的代谢产物。结论斑马鱼对组合物的代谢与大鼠或鼠肝微粒体的代谢机制高度一致,提示斑马鱼对该组合物的代谢具合理性,且具有化合物用量少、成本低、简单、高效等优点,为斑马鱼用于更复杂的中药体系代谢研究提供依据。
Objective To study the metabolism of tanshinone Ⅱ A, cryptotanshinone and tanshinone Ⅰ in zebrafish and to explore the feasibility and rationality of zebrafish in the study of multicomponent metabolism of traditional Chinese medicine. Methods The zebrafish were exposed to the pure aqueous 1% DMSO solution and detected by high performance liquid chromatography-electrospray ion trap mass spectrometry (HPLC-MS). The relative molecular mass of metabolites was obtained by quasi- Or control, the chemical composition and fish metabolites in the fish exposed to zebrafish for 24 h were identified. Results The zebrafish effect of the composition resulted in the hydroxylation and dehydrogenation of tanshinone IIA and cryptotanshinone, and no metabolites of tanshinone I were found. Conclusion The metabolic mechanism of zebrafish to the composition of rat and mouse liver microsomes is highly consistent, which indicates that the zebrafish is reasonable to metabolize the composition and has the advantages of less dosage, low cost, simplicity and high efficiency. It provided the basis for the zebrafish’s metabolism research in more complex Chinese medicine system.