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目的：了解兔实验性动脉粥样硬化（ＡＳ）进程中全血粘弹性的改变及其可能的机制。方法：高铁－硫酸显色法测定总胆固醇（Ｔｃｈ）浓度。膜蛋白含量测定用Ｌｏｗｒｙ法。膜收缩蛋白（ＳＰ）含量测定用ＳＤＳ－聚丙烯酰胺凝胶电泳法。结果：在ＡＳ进程中Ｔｃｈ浓度及ＡＧ２全血粘弹性（动粘度η’和弹性模量Ｇ’）明显升高，而钠泵活性及ＳＰ相对含量降低。Ｔｃｈ浓度与钠泵活性和ＳＰ含量间、Ｇ’与ＳＰ含量间（仅在较高角频率时）呈负相关关系，而钠泵活性与ＳＰ含量间、Ｇ’与η’间呈正相关关系。结论：Ｔｃｈ浓度的升高与钠泵活性降低间有密切关联，而它们的改变与ＳＰ含量降低有一定关系，并且上述三项因素的变化是全血粘弹性升高的重要原因
Objective: To investigate the changes of whole blood viscoelasticity and its possible mechanism during experimental atherosclerosis (AS) in rabbits. Methods: The concentration of total cholesterol (Tch) was determined by HSR - sulfuric acid method. Lowry method for the determination of membrane protein content. Membrane contractile protein (SP) content was measured by SDS-polyacrylamide gel electrophoresis. Results: Tch concentration and viscoelasticity of whole blood of AG2 (kinetic viscosity η ’and elastic modulus G’) were significantly increased in AS process, while the activity of sodium pump and the relative content of SP were decreased. There was a negative correlation between Tch concentration and sodium pump activity and SP content, between G ’and SP content (only at higher angular frequencies), while there was a positive correlation between sodium pump activity and SP content and G’ and η ’. CONCLUSION: The increase of Tch concentration is closely related to the decrease of sodium pump activity, and their changes are related to the decrease of SP content. The changes of these three factors are the important reasons for the increase of viscoelasticity of whole blood