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目的 探讨TRAPPCR ELISA间接定量法测定鼻咽癌 (NPC)中端粒酶 (TLMA)的表达。方法 采用TRAPPCRELISA法测定了 18例NPC组织、4例癌旁组织、4例慢性鼻咽炎组织及 2 2个NPCHNE1细胞株、5例白血病骨髓细胞及 2例正常骨髓细胞、6个血管平滑肌细胞株中TLMA的表达。并测定了不同浓度HNE1细胞株及NPC灭活标本中的TLMA活性。结果 NPC活检组织、癌旁组织有较高的TLMA表达 ,而慢性鼻咽炎组织为阴性 (P <0 0 1)。NPCHNE1细胞株、白血病骨髓细胞中亦有较高的TLMA表达。在 10 2 HNE1细胞株中亦能检测到TLMA活性。而 2种正常对照细胞为阴性。灭活后NPC标本中TLMA全部为阴性。结论 无论在NPC体内、体外试验中均有较高的TLMA表达。该定量法特异性好、灵敏度可达 10 2 细胞 ,具有易出结果、可靠、准确、能够反映不同程度的TLMA活性状态及与PAGE电泳图有较好的一致性等优点
Objective To investigate the expression of telomerase (TLMA) in nasopharyngeal carcinoma (NPC) by TRAPPCR indirect ELISA. Methods 18 cases of NPC tissues, 4 cases of paracancerous tissues, 4 cases of chronic nasopharyngitis and 22 cases of NPCHNE1 cell lines, 5 cases of leukemia bone marrow cells and 2 cases of normal bone marrow cells, 6 vascular smooth muscle cell lines were detected by TRAPPCRELISA. TLMA expression. The activity of TLMA in different concentrations of HNE1 cells and NPC inactivated samples was also determined. Results NPC biopsies and paracancerous tissues showed higher expression of TLMA, while chronic nasopharyngitis tissues were negative (P <0.01). NPCHNE1 cell line, leukemia bone marrow cells also have higher TLMA expression. TLMA activity was also detected in 10 2 HNE1 cell lines. The two normal control cells were negative. After inactivation of TLM NPC specimens were all negative. Conclusion Both TLMA expression and TLMA expression were higher in in vitro and in vivo experiments. The method has good specificity and sensitivity of up to 102 cells, which has the advantages of easy-out, reliable and accurate TLMA activity status and good consistency with PAGE electrophoresis