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利用限制性酶切片段长度多态性分析 (RFLP)鉴别乙型流行性感冒 (流感 )病毒的巴拿马株与维多利亚株 ,对乙型流感病毒血凝素 (HA)基因采用逆转录 聚合酶链反应 (RT PCR)扩增后 ,用HindⅢ做酶切反应并进行琼脂糖凝胶电泳。结果显示 :乙型流感病毒维多利亚株或巴拿马株HA基因均能被特异性引物所扩增 ,且与甲1与甲3 型流感病毒无交叉反应。该片段经HindⅢ酶切后 ,乙型流感病毒巴拿马株在电泳谱上出现 6 0 3bp与 2 4 2bp的 2个片段 ,而乙型流感病毒维多利亚株在该片段无 HindⅢ酶切位点 ,仍呈 872bp的一个片段。表明该方法不仅可作为常规血清学方法的补充 ,对乙型流感病毒维多利亚株与巴拿马株作出正确判定 ,而且可快速敏感地从临床标本中直接对两类乙型流感毒株进行鉴别
The Panama strains and Victoria strains of influenza B (influenza B) were identified by restriction fragment length polymorphism analysis (RFLP), and the hemagglutinin (HA) gene of influenza B virus was analyzed by reverse transcription polymerase chain reaction (RT PCR) After amplification, Hind III digestion reaction and agarose gel electrophoresis. The results showed that the HA gene of influenza B virus or Panama strain could be amplified by specific primers and had no cross-reaction with H1N1 and Influenza virus type A viruses. The fragment was digested with Hind Ⅲ, the pandemic influenza virus B of Panama appeared in the electrophoresis spectrum of 6 0 3bp and 2 4 2bp 2 fragments, and influenza B virus strains in this fragment without Hind Ⅲ restriction sites, was still A fragment of 872bp. This method can not only be used as a supplement to conventional serological methods, but also make a correct judgment on influenza B virus strains of Victoria and Panama, and can quickly and sensitively identify two types of influenza B virus strains from clinical samples directly