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最近报导,促黄体生成素释放激素(LHRH)或其高活性类似物在雌性大鼠及妇女具有抗生育作用,其作用机理之一是直接抑制性腺的功能。作者使用大鼠黄体细胞培养检查了LHRH及一种LHRH的高活性类似物(第6位D型色氨酸LHRH)抗性腺作用的机理。给未成年雌性大鼠皮下注射50IU的孕马血清促性腺激素,64小时后皮下注射25IU人绒毛膜促性腺激素。7天后处死取卵巢,用酶制备分离的黄体细胞并在适当的孵育条件下观察LHRH及其类似物对LH受体结合活性,黄体细胞孕酮生成及cAMP积累的影响。同时观察了LH、二丁酰cAMP等对LHRH抗性腺作用的影响。基于黄体细胞膜对~(125)I-HCG的结合以测定LH受体的结合。用放射免疫分析法分别测定孵育液中的cAMP、孕酮及PGF_2α。
Recently, luteinizing hormone releasing hormone (LHRH) or its highly active analogues have been shown to have anti-fertility effects in female rats and women. One of the mechanisms of action is the direct inhibition of gonadal function. The authors examined the mechanism of LHRH and a glandular response to a highly active analog of LHRH (D-tryptophan LHRH 6) using rat luteal cell cultures. Juvenile female rats were injected subcutaneously with 50 IU of pregnant mare serum gonadotropin and subcutaneously injected with 25 IU of human chorionic gonadotropin 64 hours later. After 7 days, the ovaries were sacrificed and the isolated luteal cells were prepared by enzyme. The effects of LHRH and its analogues on LH receptor binding activity, progesterone production and cAMP accumulation in luteal cells were observed under appropriate incubation conditions. At the same time, the effects of LH and dibutyryl cAMP on the glandular response to LHRH were observed. LH receptor binding was determined based on the binding of luteal cell membranes to ~ (125) I-HCG. Radioimmunoassay was used to determine the cAMP, progesterone and PGF 2α in the incubation solution respectively.