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对本研究室自建的小鼠前胃癌（ＭＦＣ）细胞系、株和小鼠树突状细胞肉瘤（ＤＣＳ）细胞系、株的移植瘤组织和培养细胞进行ｎｍ２３／ＮＤＰＫ活性、免疫组化染色及ＲＮＡ杂交的研究。－３２ＰＵＴＰ生成法和乳酸脱氢酶一丙酮酸激酶偶联法均证明这些瘤组织或瘤细胞存在ＮＤＰＫ活性。ＭＦＣ、株转移率高者ＮＤＰＫ活性低，反之则高；而ＤＣＳ系、株瘤组织ＮＤＰＫ活性高、低与瘤株转移率高、低无明显规律性关系。ＭＦＣ和ＤＣＳ各株细胞的ＮＤＰＫ免疫组化染色呈弱阳性，ｎｍ２３ＲＮＡ狭缝杂交（地高辛标记探针）均呈阴性。结果提示ｎｍ２３／ＮＤＰＫ对ＭＦＣ可能具有转移抑制的功能，而对ＤＣＳ可能无明显抑制作用
The nm23/NDPK activity and immunohistochemical staining of the mouse precancerous gastric (MFC) cell line, strain and mouse dendritic cell sarcoma (DCS) cell line, tumor xenograft tissue and cultured cells were established in our laboratory. RNA hybridization studies. The -32PUTP generation method and the lactate dehydrogenase-pyruvate kinase conjugation method both demonstrated the presence of NDPK activity in these tumor tissues or tumor cells. The activity of NDPK was higher in MFC and higher metastasis rate, but higher in the contrary, while the activity of NDPK was higher in DCS system and tumor tissue and the metastasis rate of tumor strain was high and low. The NDPK immunohistochemical staining of MFC and DCS cells was weakly positive, and the nm23 RNA slit hybridization (digose-labeled probe) was negative. The results suggest that nm23/NDPK may have a metastasis-inhibitory effect on MFC but may not have a significant inhibitory effect on DCS.