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目的研究急性髓系白血病细胞HL-60诱导分化后抗原处理相关转运体蛋白(TAP)及低分子量蛋白酶体(LMP)在该细胞模型中的表达变化及其意义。方法本实验分对照组、ATRA诱导组和PMA诱导组;Wright-Gimesa染色观察HL-60细胞形态,流式细胞术检测细胞表面标记CD11b、CD14的表达,RT-PCR检测LMP/TAP在mRNA水平的表达,Western blotting检测细胞TAP2基因的蛋白表达。结果 0.8μmol/L全反式维甲酸(ATRA)和50 nmol/L乙酸肉豆蔻佛波酯(PMA)分别作用HL-60细胞72 h后可显著抑制细胞增殖,并诱导细胞向成熟粒细胞及巨噬细胞方向分化;CD11b和CD14表达水平均明显升高(P均<0.05);TAP/LMP的mRNA表达水平及TAP2的蛋白表达水平均升高(P均<0.05)。结论白血病来源的单核样细胞具有一定的免疫功能表型,为进一步研究LMP/TAP在白血病免疫逃逸中的作用奠定基础。
Objective To study the expression and significance of antigen-related transporter protein (TAP) and low molecular weight proteasome (LMP) in acute myeloid leukemia cell line HL-60 after differentiation. Methods The experimental group was divided into control group, ATRA induction group and PMA induction group. Wright-Gimesa staining was used to observe the morphology of HL-60 cells. Flow cytometry was used to detect the expression of CD11b and CD14. RT- The protein expression of TAP2 gene was detected by Western blotting. Results After HL-60 cells were treated with 0.8 μmol / L ATRA and 50 nmol / L PMA for 72 h, the proliferation of HL-60 cells was significantly inhibited and the cells were induced to mature granulocytes and (P <0.05). The mRNA expressions of TAP / LMP and TAP2 were all increased (all P <0.05). Conclusion Leukemia-derived mononuclear cells have a certain immune phenotype, which lays the foundation for further study on the role of LMP / TAP in leukemia immune escape.