论文部分内容阅读
目的体外培养大鼠骨髓间充质干细胞(MSCs),探讨其对移植肾的保护作用。方法无菌条件下取大鼠股骨、胫骨,用直接贴壁法分离纯化MSCs,体外扩增,在注射MSCs的基础上,行同种异体肾移植,实验大鼠分为注射大鼠骨髓MSCs肾移植组(Ⅰ组)和单纯肾移植组(Ⅱ组),环孢素A治疗肾移植组(Ⅲ组)术后5 d结扎受体右肾动脉(左侧为移植肾);观察右肾动脉结扎术后受体存活时间,检测右肾术后移植肾的形态学变化,肌酐水平。结果Ⅰ组、Ⅲ组大鼠生存期(15.50±6.35)d和(18.50±5.97)d较Ⅱ组(6.50±3.11)d明显延长,且前者移植肾形态学观察和功能状态均明显优于后者。结论骨髓间充质干细胞在体外很容易分离培养和扩增,骨髓间充质干细胞对早期移植肾具有保护作用。
OBJECTIVE: To culture rat bone marrow mesenchymal stem cells (MSCs) in vitro and investigate their protective effect on renal allograft. Methods The aseptic conditions of rat femur and tibia were isolated and purified by direct adherent MSCs, expanded in vitro, MSCs were injected on the basis of allograft kidney transplantation, the experimental rats were divided into rat bone marrow MSCs The rats in the transplantation group (group Ⅰ) and the simple kidney transplantation group (group Ⅱ) and the cyclosporin A-treated kidney transplantation group (group Ⅲ) were ligated to the recipient right renal artery (left kidney) 5 days after the operation. The right renal artery The survival time of the recipients after ligation was measured. Morphological changes and creatinine of the transplanted kidneys were measured. Results The survival time of group Ⅰ and group Ⅲ was significantly longer than that of group Ⅱ (6.50 ± 3.11) d for (15.50 ± 6.35) days and (18.50 ± 5.97) days, and the morphological observation and functional status of the former were superior to the latter By. Conclusion Bone marrow mesenchymal stem cells can be easily isolated and cultured in vitro. Bone marrow mesenchymal stem cells have a protective effect on early graft kidney.