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目的:为探讨缺血-再灌注(IR)对移植肺损伤的可能机制,建立大鼠在体肺低温IR损伤模型。方法:模拟临床肺移植建立大鼠在体肺低温IR损伤模型。分别于游离左肺门(对照组,A组)、缺血4h(缺血组,B组)和缺血4h再灌注4h(IR组,C组)后测定动脉血氧分压(PaO2)、动脉血二氧化碳分压(PaCO2)、气道峰压(Paw)以及支气管肺泡灌洗液(BALF)中蛋白含量和左肺含水量,并取左肺组织作病理学观察。结果:所有动物术后全部存活。PaO2、Paw、BALF蛋白含量及左肺含水量,C组与A组及B组之间的差异显著(P<0.01);三组间PaCO2无明显差异(P>0.05);肺病理改变以C组最严重。结论:该方法的建立为研究IR对移植肺的损害机制提供了较为理想的动物模型。
OBJECTIVE: To investigate the possible mechanism of ischemia-reperfusion (IR) on lung injury in rats and to establish a model of IR injury in vivo. Methods: To establish a model of lung injury induced by IR in vivo. The arterial partial pressure of oxygen (PaO2) was measured in free left hilar (control group, group A), ischemia 4h (ischemia group, group B) and ischemia 4h after reperfusion for 4h PaCO2, Paw and protein in BALF and left lung water content of left lung were observed. Results: All animals survived postoperatively. PaO2, Paw, BALF protein content and left lung water content, the difference between C group and A group and B group was significant (P <0.01); There was no significant difference in PaCO2 among the three groups (P> 0.05) The most serious group. Conclusion: The establishment of this method provides an ideal animal model for studying the mechanism of IR damage to lung transplantation.