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目的:探索中脑黑质多巴胺神经元对适当剂量鱼藤酮毒性损伤是否具有特殊敏感性。方法:采用颈背部皮下注射鱼藤酮的方法建立大鼠中脑黑质多巴胺神经元损伤模型,进行中脑黑质和纹状体酪氨酸羟化酶(TH)免疫组织化学染色加尼氏染色;同时通过HE染色及尼氏染色方法分别观察心、肝、脾、肾等重要胸腹腔脏器及海马、顶叶皮质的形态学变化。结果:中脑黑质TH免疫染色和尼氏染色结果显示组间中脑黑质致密区以外部位尼氏小体数目无差异;大脑顶叶皮质和海马尼氏染色及胸腹腔重要脏器HE染色结果表明各组大鼠均未出现相应部位损伤。结论:低剂量颈部皮下注射鱼藤酮能选择性诱导中脑黑质多巴胺神经元损伤,说明中脑黑质多巴胺神经元对鱼藤酮具有高度敏感性。
Objective: To explore whether midbrain substantia nigra dopamine neurons have special sensitivity to the toxic dose of rotenone. Methods: The rat model of substantia nigra dopamine neurons injury was established by subcutaneous injection of rotenone on the back of the neck. Tyrosine hydroxylase (TH) immunohistochemical staining of the midbrain substantia nigra and striatum was performed. At the same time, the morphological changes of important thoracoabdominal organs, hippocampus and parietal cortex in heart, liver, spleen and kidney were observed by HE staining and Nissl staining respectively. Results: TH immunostaining and Nissl staining of substantia nigra in the midbrain showed no difference in the number of Nissl body outside the substantia nigra in the midbrain. Nissl staining of the parietal cortex and hippocampus and HE in the vital organs of the thoracoabdominal cavity The results showed that each group of rats did not appear the corresponding part of the injury. Conclusion: Subcutaneous injection of rotenone on the lower cervical neck can selectively induce dopaminergic neuron injury in substantia nigra of midbrain, indicating that dopamine neurons in mesencephalic substantia nigra are highly sensitive to rotenone.