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在pH4.5～7.0的Britton-Robinson(BR)缓冲溶液中,呋塞米(FUR)与Pd(Ⅱ)形成1:1的螯合阴离子,它能进一步与乙基紫(EV)、结晶紫(CV)、甲基绿(MeG)、亮绿(BG)、甲基紫(MV)等碱性三苯甲烷染料(BTPMD)阳离子通过静电引力和疏水作用形成FUR:Pd(II):BTPMD为1:1:1的离子缔合物.此时,该离子缔合反应不仅能引起吸收光谱的变化,而且更能导致共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)的显著增强,其最大RRS波长分别位于324nm(EV,CV和MV体系)和340nm(BG和MeG体系),最大SOS波长分别位于550nm(EV,CV,BG和MeG体系)和530nm(MV体系),而最大FDS波长均位于392nm附近.在一定条件下三种散射增强(ΔIRRS,ΔISOS和ΔIFDS)均与呋塞米(FUR)的浓度成正比.对不同染料体系,三种方法对FUR的检出限分别在0.3～4.9ng/mL(RRS),3.2～33.1ng/mL(SOS)和9.0～85.7ng/mL(FDS)之间,均可用于痕量FUR的测定.本文研究了三元离子缔合物的形成对吸收,RRS,SOS和FDS光谱特征和强度的影响,考察了适宜的反应条件、影响因素和分析化学性质,并以RRS法为例考察了共存物质的影响.据此提出了一种高灵敏度、简便、快速测定FUR的共振光散射新方法,将其用于片剂、注射液、人血清和尿样中FUR的测定,结果满意.文中还对三元离子缔合物的组成、结构和反应机理进行了讨论.
Furosemide (FUR) forms a 1: 1 chelating anion with Pd (Ⅱ) in Britton-Robinson (BR) buffer solution at pH 4.5-7.0, which can further interact with ethyl violet (EV), crystal violet FUR: Pd (II): BTPMD is formed by electrostatic attraction and hydrophobic interaction with basic triphenylmethane dye (BTPMD) such as CV, MeG, BG and MV. 1: 1: 1 ion association. At this time, the ion association reaction can not only lead to changes in absorption spectra, but also lead to Resonance Rayleigh Scattering (RRS), secondary scattering (SOS) and frequency doubling scattering FDS). The maximum RRS wavelengths are located at 324nm (EV, CV and MV systems) and 340nm (BG and MeG systems) respectively. The maximum SOS wavelengths are located at 550nm (EV, CV, BG and MeG systems) System), and the maximum FDS wavelength is near 392nm. Under certain conditions, the three kinds of scattering enhancement (ΔIRRS, ΔISOS and ΔIFDS) are proportional to the concentration of furosemide (FUR) .For different dye systems, (FDS) of 0.3 ~ 4.9ng / mL (RRS), 3.2 ~ 33.1ng / mL (SOS) and 9.0 ~ 85.7ng / mL Formation of ternary ion association to absorption, RRS, SOS FDS spectroscopy and intensity, the appropriate reaction conditions, influencing factors and analytical chemistry properties were investigated, and the influence of coexisting substances was investigated by taking RRS method as an example. A high sensitivity, simple and rapid determination of FUR Resonance light scattering method was used to determine the FUR in tablets, injection, human serum and urine samples with satisfactory results.The composition, structure and reaction mechanism of ternary ion-association complexes were also discussed.