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目的：探讨血清甘氨酰脯氨酸二肽氨基肽酶（ＧＰＤＡ）活性测定对胃癌的诊断价值及方法学改良。方法：速率法（酶法）检测良恶性胃病患者的ＧＰＤＡ活性。结果：胃癌（无转移）组２５．３３±６．８５Ｕ／Ｌ；胃癌（肝转移）组１５６．９２±１４．６５Ｕ／Ｌ；胃溃疡组７６．８１±１１．５２Ｕ／Ｌ；正常对照组７８．１９±１０．９７Ｕ／Ｌ。胃癌无转移的ＧＰＤＡ活性显著低于正常对照组及胃溃疡组（Ｐ＜０．０１），但胃癌伴肝转移者却显著高于其它各组（Ｐ＜０．０１）。本法线性良好，批内变异系数（ＣＶ）分别为１．９８％、１．２６％、２．１８％；批间ＣＶ分别为２．３５％、２．０９％、３．１８％；经对比试验相关性良好。结论：本法速度快，精度高。ＧＰＤＡ可作为早期胃癌诊断的指标之一，并能对胃癌的肝转移进行有效的监控。
Objective: To investigate the diagnostic value and methodological improvement of serum glycyl-proline dipeptide aminopeptidase (GPDA) activity in gastric cancer. Methods: The rate of GPDA activity in patients with benign and malignant gastropathy was measured by enzymatic method. Results: The gastric cancer (no metastasis) group was 25.33±6.85 U/L; the gastric cancer (liver metastasis) group was 156.92±14.65 U/L; the gastric ulcer group was 76.81±11.52 U/L; the normal control group 78.19±10.97 U/L. The GPDA activity of gastric cancer without metastasis was significantly lower than that in the normal control group and the gastric ulcer group (P<0.01), but the gastric cancer with liver metastasis was significantly higher than other groups (P<0.01). This method has a good linearity, with intra-assay coefficient of variation (CV) of 1.98%, 1.26%, and 2.18%, inter-assay CV of 2.35%, 2.09%, and 3.18%, respectively; The comparative test has good correlation. Conclusion: This method is fast and accurate. GPDA can be used as one of the indicators of early gastric cancer diagnosis, and can effectively monitor the liver metastasis of gastric cancer.