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本研究以博落回总生物碱含量及回收率等为考察指标,研究大孔吸附树脂分离纯化博落回总生物碱工艺。结果表明:AB-8型大孔吸附树脂对博落回总生物碱静态饱和吸附量为104.65mg/g(干树脂),洗脱率95.9%,动态饱和吸附量为96.5mg/g(干树脂),总生物碱回收率在91.24%、纯度在90%以上,是实验树脂中分离纯化博落回总生物碱的最佳大孔吸附树脂。分离纯化博落回总生物碱最佳工艺条件为:AB-8型大孔吸附树脂,洗脱剂为90%乙醇,洗脱剂用量为2～3倍树脂体积,上柱总生物碱量与树脂比为1:10.4,上柱液总生物碱浓度为21.57mg/ml,流速2～3ml/min,上柱液pH值7～8。
In this study, the total alkaloids content and recovery rate of Bo and back as index to study the separation and purification of Macroporous Adsorption Polygonum total alkaloids process. The results showed that the adsorption capacity of AB-8 macroporous resin was 104.65mg / g (dry resin), the elution rate was 95.9% and the dynamic saturated adsorption capacity was 96.5mg / g (dry resin ), The total alkaloid recovery was 91.24% and the purity was above 90%. It was the best macroporous adsorption resin for separation and purification of total alkaloids from Fructus Lycii in the experimental resin. The optimum conditions for the separation and purification of the total alkaloids from P. dahurica were as follows: AB-8 macroporous adsorption resin, 90% ethanol as eluent, 2 ~ 3 times as volume of eluent, Resin ratio of 1: 10.4, the total alkaloid concentration on the column was 21.57mg / ml, the flow rate of 2 ~ 3ml / min, on the column solution pH 7 ~ 8.