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浅黄恩蚜小蜂Encarsia sophia(Hymenoptera:Aphelinidae)是一种有潜在应用价值的烟粉虱寄生蜂,在实验室条件下,浅黄恩蚜小蜂与丽蚜小蜂Encarsia formosa(Hymenoptera:Aphelinidae)共同存在时,表现出竞争优势。目前还很难准确判断早期被蚜小蜂寄生的烟粉虱,因此寻找一种有效快速鉴定烟粉虱的寄生蜂早期寄生的方法非常重要。本研究发现在检测烟粉虱寄生蜂早期寄生的每个阶段(卵、1龄和2龄幼虫)时,基于线粒体DNA细胞色素氧化酶COI基因的PCR方法比同工酶电泳的更灵敏,同工酶电泳只能在寄生蜂发育至3龄及以上时才能检测出烟粉虱的寄生情况。PCR方法同时也能够区分早期发育阶段的浅黄恩蚜小蜂和丽蚜小蜂。本研究所建立的PCR方法快速、灵敏、可靠,可代替同工酶电泳和解剖的方法,用于烟粉虱早期寄生的鉴定,从而实现实验室和田间烟粉虱寄生率的快速评估。
Encarsia sophia (Hymenoptera: Aphelinidae), a potential parasitic wasp of Bemisia tabaci, is co-cultured with Encarsia formosa (Hymenoptera: Aphelinidae) under laboratory conditions When present, it shows a competitive advantage. It is very difficult to accurately determine the whiteflies that have been parasitized by the aphid insects in the early stage. Therefore, it is very important to find an effective method for early identification of parasitoid parasites that can effectively identify whitefly. This study found that the PCR method based on the COI gene of mitochondrial DNA cytochrome oxidase is more sensitive than the isozyme electrophoresis in detecting each stage (parasitoid, 1st and 2nd instar larvae) of the parasitic wasps of the Bemisia tabaci Enzymatic electrophoresis can only detect the parasitic status of B. tabaci when the parasitic wasps develop to 3rd instar and above. PCR method also can distinguish between early development stages of the yellow aphid wasps and ragweed wasps. The PCR method established in this study is a rapid, sensitive and reliable method that can replace the isozyme electrophoresis and dissection method for the identification of the early parasitism of Bemisia tabaci (Gennadius) so as to achieve a rapid assessment of the parasitism rate of Bemisia tabaci in laboratory and field.