论文部分内容阅读
目的 探讨口服不同剂量的谷胱甘肽 (GSH)对氟所致大鼠脂质过氧化的影响。方法 对事先饮用 10天 15 0mg/L 氟化钠 (Na F)的雄性 Wistar大鼠分别给予 6 0、30 0和 6 0 0 mg/kg GSH灌胃 12周 ,同时设对照组 (饮蒸馏水 )和单纯氟染毒组 (15 0 mg/L Na F) ,测定血清 (全血 )、肝、肾、心、睾丸和脑组织中超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量。结果 单纯氟染毒可使大鼠血清、肝、肾、脑 MDA含量显著增加 ,全血、肝、肾、心、睾丸 SOD活性显著降低 ;口服GSH后 ,血清、肝、肾、脑 MDA的生成减少 ,肝、肾、心、睾丸和 SOD活性显著上升 ,同时 30 0 mg/kg GSH可显著促进尿氟的排出。结论 氟可使大鼠脂质过氧化作用增强 ,抗氧化能力降低 ,口服 GSH可拮抗氟致脂质过氧化作用 ,增加抗氧化能力。
Objective To investigate the effects of oral administration of different doses of glutathione (GSH) on lipid peroxidation induced by fluorine in rats. Methods Male Wistar rats, who had been drinking 150 mg / L sodium fluoride (NaF) for 10 days, were administered with GSK (60,30, and 60 mg / kg) for 12 weeks respectively. At the same time, And pure fluorine (15 0 mg / L NaF) were measured respectively. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum (whole blood), liver, kidney, heart, . Results Pure fluorine exposure could significantly increase the content of MDA in serum, liver, kidney and brain, and decrease the activity of SOD in whole blood, liver, kidney, heart and testis. After oral administration of GSH, MDA, Decrease, liver, kidney, heart, testis and SOD activity increased significantly, while 30 0 mg / kg GSH can significantly promote the urinary fluoride excretion. Conclusion Fluoride can enhance the lipid peroxidation in rats and decrease the anti-oxidation ability. Oral administration of GSH can antagonize the lipid peroxidation induced by fluoride and increase the antioxidant capacity.