巴西利什曼原虫是引起南美、中美大部和北美某些地区的局限性皮肤利什曼病和粘膜皮肤利什曼病的病原。为探索更好的种和亚种的鉴别诊断探针,本文作者研究了基因组重复DNA序列。 感染人类的利什曼原虫株分离自委内瑞拉皮肤利什曼病患者,在含10％去纤维蛋白兔血的琼脂培养基中24℃培养,离心、裂解 Leishmania braziliensis is the causative agent of localized cutaneous leishmaniasis and mucocutaneous leishmaniasis in South America, most of Central America and some parts of North America. To explore differential diagnosis probes for better species and subspecies, the authors studied genomic repeat DNA sequences. Human leishmania strains were isolated from patients with skin cutaneous leishmaniasis in Venezuela, cultured in an agar medium containing 10% defibrinated rabbit blood at 24 ° C, centrifuged, lysed