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为研究大豆异黄酮和皂甙对D-氨基半乳糖(GalN)所致小鼠急性肝损伤的保护作用以及对肝脏抗氧化活力的影响,将试验小鼠随机分为正常组、模型组、大豆异黄酮组、大豆皂甙组及联苯双脂组(阳性对照组)。每日给药1次,连续7 d。试验末期,腹腔注射GalN建立小鼠急性肝损伤模型,比色法检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)以及肝脏过氧化脂质(LOOH)、丙二醛(MDA)、还原型谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)活性。结果表明:大豆异黄酮和皂甙降低GalN所致急性肝损伤小鼠血清ALT和AST活性,增高血清ALB水平,减少肝LOOH和MDA含量,升高肝组织GSH水平和SOD、CAT、GPx活性。大豆异黄酮和皂甙对GalN所致小鼠急性肝损伤具有保护作用,其机制可能与抗氧化作用有关。
In order to study the protective effect of soy isoflavones and saponins against acute liver injury induced by D-galactosamine (GalN) in mice and its effect on liver antioxidant activity, the mice were randomly divided into normal group, model group, Flavonoids group, soybean saponin group and biphenyl two lipid group (positive control group). Administered once a day for 7 days. At the end of the experiment, the model of acute liver injury was established by intraperitoneal injection of GalN. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB) and liver lipid peroxidation (LOOH), malondialdehyde (MDA), reduced glutathione (GSH) and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity. The results showed that soy isoflavones and saponins could decrease ALT and AST, increase serum ALB, decrease LOOH and MDA contents, increase GSH, SOD, CAT and GPx activity in liver of mice with acute liver injury induced by GalN. Soy isoflavones and saponins have a protective effect on GalN-induced acute liver injury in mice, the mechanism may be related to anti-oxidation.