以往有关线粒体的研究,一般是将线粒体分离出来加以测定。但对含硅质较多的水稻叶片,欲获得完整的离体线粒体,分离技术比较复杂。本实验成功地用原位固定法,借助电镜观察了水稻叶片衰老期间线粒体结构的变化。1 材料与方法水稻幼苗在人工光照室内水培。生长10天后待第二片叶片完全展开,取5厘米长叶尖,投入盛水的三角瓶内,分别进行光照和黑暗处理。继在距叶尖2厘米处切取2毫米长叶段若 Previous research on mitochondria, the mitochondria are generally isolated to be measured. However, for the more silicon-containing rice leaves, to obtain complete mitochondria in vitro, the separation technique is more complicated. In this experiment, we successfully observed the changes of mitochondrial structure during the senescence of rice leaves by using in situ fixation. 1 Materials and Methods Rice seedlings were hydroponic cultured in artificial light. After the growth of 10 days to be the second leaf fully expanded, take 5 cm long tip, into the water flask, were light and dark treatment. Following 2 cm away from the tip of the cut 2 mm long leaves if