杂交水稻细胞质效应研究——叶绿体、线粒体超微结构的观察

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电子显微镜观察了雄性败育的野生稻“野败”与雄性育性正常的栽培稻“Gamb-iaKa”和以它们为质供体育成的不育系W-珍汕97A、G-29矮A,相应的保持系、恢复系、不育系×恢复系F_1、保持系×恢复系F_1等,12份水稻材料的剑族叶叶肉细胞中叶绿体和线粒体的超微结构。结果表明:1.两种类型的质供体育成的不育系都表现出线粒体内嵴发育不完全,内部出现很大的空洞;叶绿体不同基粒间的片层排列方向不一致,呈现一定程度的杂乱。这两种异常现象可能与水稻的雄性不育性相联系。但是,不育系的叶绿体基粒片层数未见变化。2.当不育系与恢复系杂交后,F_1的线粒体和叶绿体全部从原来的异常现象恢复正常,并且更为发达,基本上类似保持系和恢复系杂交F_1线粒体和叶绿体。3.野败叶绿体基粒片层数少,珍汕97B的片层数多,但两者的质与核共处了23代之久的珍汕97A,其片层数未见变化,当不育系和恢复系杂交后,F_1代的片层数却大为增加。可见,水稻的质核关系应做具体分析。 Electron microscope observation of male abortion wild rice “wild defeat” and male fertility normal cultivated rice “Gamb-iaKa” and their quality for the male sterile line of sterile W-Zhenshan 97A, G-29 short A , The corresponding maintainer line, restorer line, male sterile line × restorer line F_1, maintainer line × restorer line F_1, etc. The ultrastructures of chloroplast and mitochondria in 12 Glycine max. The results showed that: 1. Two types of sterile CMS lines showed incomplete development of mitochondrial inner cristae and large internal cavities. The lamellar arrangement directions of chloroplasts differed from each other to a certain extent Messy These two anomalies may be linked to male sterility in rice. However, there was no change in the number of chloroplast grains in CMS lines. When the male sterile line and the restorer line were crossed, all the F_1 mitochondria and chloroplast were normalized from the original anomaly, and more developed, basically similar to the F_1 mitochondria and chloroplast of maintainer line and restorer line. 3. The number of wild-leaf chloroplast grana lamellae was less than that of Zhenshan 97B. However, the quality and nucleus of the two coexisted for 23 generations and the number of Zhenshan 97A had no change. When infertile After the lines were crossed with the restoring lines, the number of F_1 generations was greatly increased. Can be seen that the relationship between the quality of rice should be done specifically.
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