论文部分内容阅读
造血干细胞与血液系统疾病有密切关系。利用骨髓造血干细胞培养,对各类血液病的病因、发病机理的探讨、临床诊断及疗效和预后的观察都有重要意义。1965年Pluznick 等首先报告用小鼠骨髓细胞或血细胞,在集落刺激因子(下称 CSF)作用下,在半固体琼脂培养基上,形成粒细胞和单核-巨噬细胞组成的集落(下称 CFU-C)。1970年 Pike 等进一步研究了人的骨髓细胞体外软琼脂培养技术,并成功地培养了人
Hematopoietic stem cells and blood diseases are closely related. The use of bone marrow hematopoietic stem cell culture, the etiology and pathogenesis of various types of blood diseases, clinical diagnosis and efficacy and prognosis are of great significance. In 1965, Pluznick et al. First reported that using mouse bone marrow cells or blood cells, colony formed by granulocytes and mononuclear macrophages under the action of colony stimulating factor (hereinafter referred to as CSF) on semi-solid agar medium CFU-C). In 1970, Pike et al further studied the in vitro soft agar culture of human bone marrow cells and successfully cultivated human