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目的 观察ＩＬ１ 对成纤维细胞生长的影响及其作用机理，为从细胞因子角度开展抗纤维化研究提供依据。方法 用ＭＴＴ比色、ＡｇＮＯＲＳ 银染和免疫组化技术，观察正常和受照ＮＩＨ／３Ｔ３ 成纤维细胞（６０Ｃｏγ源一次照射，吸收剂量３０ Ｇｙ）生长增殖活性、ＡｇＮＯＲＳ 和ＤＮＡ合成以及Ⅲ型胶原表达。结果 ＩＬ１ 浓度为１００ ×１０３Ｕ／Ｌ，培养第３ 天时，成纤维细胞数量和增殖活性（Ａ 值） 为对照值的１－４ 和１－３ 倍（照射组Ａ值为对照的１－１ 倍）；ＡｇＮＯＲＳ 颗粒数和Ｓ期细胞比例分别升高至对照值的２－９ 和２－０ 倍；而Ⅲ型胶原表达阳性细胞数为对照值的１－９ 倍。结论 在一定浓度范围内，ＩＬ１ 能促进成纤维细胞的增殖活性，ＤＮＡ 合成和Ⅲ型胶原表达。
Objective To observe the effect of IL1 on the growth of fibroblasts and its mechanism of action, to provide a basis for the study of antifibrosis from the perspective of cytokines. Methods MTT colorimetry, AgNORS silver staining and immunohistochemistry were used to observe the proliferation and proliferation of normal and irradiated NIH / 3T3 fibroblasts exposed to a single irradiation of 60Coγ ray with a dose of 30 Gy, AgNORS and DNA synthesis, and type Ⅲ collagen expression . Results The concentration of IL1 was 100 × 103U / L, the number of fibroblasts and the proliferative activity (A value) were 1-4 and 1-3 times of the control on the third day after culture (the value of A in control group was 1- 1 times). The number of AgNORS particles and the proportion of S phase cells increased to 2-9 and 2-0 times of the control respectively. The number of type Ⅲ collagen-positive cells was 1-9 times that of the control. Conclusion In a certain concentration range, IL 1 can promote fibroblast proliferation activity, DNA synthesis and type Ⅲ collagen expression.