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目的:以ITS2片段作为DNA条形码,在分子水平上研究白果五味子。方法:结合五味子和华中五味子样品对白果五味子进行研究,对样品ITS2片段进行双向测序,CodonCode Aligner拼接序列,利用MEGA 5.0进行多序列比对,利用ITS2数据库及其网站预测ITS2二级结构,应用BLAST 1方法进行鉴定分析。结果:白果五味子样品ITS2序列长度为231 bp,BLAST 1鉴定白果五味子样品为五味子。白果五味子和五味子居群间平均K2P(Kimura-2-parameter)遗传距离(0.002 2)远远小于五味子和华中五味子的种间平均K2P遗传距离(0.021 6)。结论:ITS2鉴定白果五味子属于五味子;ITS2可以用于鉴定和区分五味子和华中五味子。
OBJECTIVE: To study the Ginkgo bilobica at molecular level by using ITS2 fragment as DNA barcode. Methods: Combining the Schisandra chinensis and Schisandra chinensis samples, we studied the Schisandra chinensis fruit by bi-directional sequencing of the ITS2 fragment, CodonCode Aligner splicing sequence, MEGA 5.0 multiple sequence alignment, ITS2 database and its website to predict the ITS2 secondary structure. BLAST 1 method for identification analysis. Results: ITS2 sequence length of Ginkgo biloba sample was 231 bp. BLAST 1 was used to identify the Ginkgo biloba sample. The average k2 (Kimura-2-parameter) genetic distance (0.002 2) between the populations of Schisandra chinensis and Schisandra was significantly smaller than that between the Schisandra chinensis and Schisandra chinensis populations (0.021 6). Conclusion: ITS2 identification Schisandra Schisandra belongs to Schisandra; ITS2 can be used to identify and distinguish Schisandra and Schisandra chinensis.