对白痢沙门菌二元调控系统响应蛋白CpxR进行生物信息学分析,提示CpxR可能发挥了重要的生理功能。同时,构建了重组表达载体pET32a-CpxR,转化表达菌株BL21后,对其进行诱导表达,SDS-PAGE和Western blot结果表明CpxR获得了正确表达。纯化蛋白免疫小鼠制备了多抗血清,Western blot验证其有一定的免疫原性。为深入研究其生理功能和调控机制奠定了理论基础。 Bioinformatics analysis of the CpxR response protein of Salmonella pullorum binary system suggested that CpxR may play an important physiological function. At the same time, the recombinant expression vector pET32a-CpxR was constructed and transformed into E.coli BL21 for expression under induction. The results of SDS-PAGE and Western blot showed that CpxR was correctly expressed. The purified protein was used to immunize mice to prepare multiple antiserum, which was proved to be immunogenic by Western blot. Which lays a theoretical foundation for further study of its physiological functions and regulatory mechanisms.