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目的:探讨常规体外受精-胚胎移植(IVF-ET)受精失败后,对未受精卵母细胞进行补救性单精子卵胞浆内注射(ICSI)治疗的可行性和安全性。方法:超促排卵获得的卵子进行常规体外受精,16小时后对全部或>70%卵母细胞未受精的病例,进行补救ICSI,所获得的优质胚胎进行宫腔内移植。结果:7例患者51枚未受精卵母细胞行补救ICSI后,受精37枚,卵裂30枚,优质胚胎17个,受精率72.5%,卵裂率81%,与20例单纯行ICSI治疗者比较,受精率、卵裂率无统计学显著性差异。补救ICSI组1例获临床妊娠,并分娩健康新生儿,随访1年余未发现异常,临床妊娠率(14.3%)较单纯ICSI组(35%)显著降低(P<0.01)。结论:常规体外受精-胚胎移植受精失败后,对未受精卵母细胞进行补救性ICSI,有机会获得健康新生儿。
Objective: To investigate the feasibility and safety of intracytoplasmic sperm injection (ICSI) for unfertilized oocyte after the failure of conventional in vitro fertilization-embryo transfer (IVF-ET). Methods: Ovules obtained by superovulation were routinely fertilized in vitro. ICSI was performed on all or> 70% of oocytes without fertilization after 16 hours, and the obtained high quality embryos were transplanted into uterine cavity. Results: After immunosuppression of 51 ICSIs in 7 patients, 37 fertilized ICSIs, 30 cleavage tips, 17 high quality embryos, fertilization rate 72.5% and cleavage rate 81% were obtained from 51 unfertilized oocytes. Compared with 20 ICSI patients Comparison, fertilization rate, cleavage rate no statistically significant difference. One patient in the ICSI group was clinically pregnant and gave birth to a healthy newborn. No abnormality was found over a year of follow-up. The clinical pregnancy rate (14.3%) was significantly lower than that of the ICSI group (35%) (P <0.01). CONCLUSIONS: Conventional in vitro fertilization-embryo transfer fails to rescue ICSI for unfertilized oocytes, giving them the opportunity to obtain healthy newborns.