实验中,有时需要计数和比较角膜的上皮细胞数目,计数方法尚未见报道。现介绍一种离体角膜的上皮细胞计数方法。沿角膜缘剪下完整角膜,按扫描电镜样本常规制样。角膜接近于圆形,计数角膜上皮细胞时可将角膜看作圆形。扫描电镜下放大15倍时,基本可见整个角膜 In experiments, it is sometimes necessary to count and compare the number of corneal epithelial cells, counting methods have not been reported. Now introduced a corneal epithelial cell counting method. Corneal edge along the cut intact cornea, according to the SEM sample preparation. The cornea is nearly circular, and the cornea can be considered as a circle when counting corneal epithelial cells. Scanning electron microscope enlarged 15 times, the basic visible cornea