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蔬菜灰霉病菌(Botrytis cinerea)分生孢子经紫外光灯照射,可获得10~(-4)～10~(-5)频率的腐霉利抗药性突变体。获得的13个抗性突变体的抗性水平平均EC_(50)值为18.45μg/ml,EC_(90)值为305.17μg/ml。抗性突变体的可溶性蛋白谱带比其亲本敏感菌株多2～4条,其中Rf=0.38的蛋白谱带是抗性菌株所拥有而敏感菌株所共同缺乏的。抗性菌株对腐霉利抗药性稳定遗传,具有与亲本菌株相同的致病性。苗期抗、感测定结果表明,灰霉克杀菌剂对腐霉利突变体具有明显的克抗作用。田间抗性菌治理使用灰霉克400～800倍对大棚番茄灰霉病平均防效为95.93％～75.8％,优于腐霉利600倍的平均防效(61.32％)。
The conidia of Botrytis cinerea were irradiated with UV light, and the mutagen resistant mutants of 10 -4 to 10 -5 were obtained. The resistance of the 13 resistant mutants obtained showed an average EC 50 value of 18.45 μg / ml and an EC 90 value of 305.17 μg / ml. The soluble protein bands of the resistant mutants were 2 to 4 more than their parentally sensitive strains, and the protein band with Rf = 0.38 was common to the resistant strains but not to the sensitive strains. The resistant strains were stably inherited to the resistant strain of Pythium, with the same pathogenicity as the parent strain. Seedling anti-sense test results show that gray mold g fungicide on the susceptibility of the mutant of the goethomycone obvious role. The average control efficiency of gray mold from 400 to 800 times against tomato gray mold in greenhouse was 95.93% ~ 75.8%, which was superior to that of Pythium 600 times (61.32%).