论文部分内容阅读
目的观察绿色荧光灯对大鼠视网膜光化学损伤的动态变化过程。方法采用（１９００±１０６．９）Ｌｘ绿色荧光灯对ＳＤ大鼠进行持续２４小时照射，分别于光照前，光照后６小时、６天及１４天进行视网膜光镜形态学观察、外核层厚度及ＦＥＲＧ检测。结果光照前视网膜形态正常，结构层次清楚，内外节排列整齐规则。光照后６小时视网膜外核层变薄，其厚度减少２３．９１％，内外节排列紊乱。光照后６天外核层更薄，其厚度减少４６．６０％。１４天外核层较６天增厚，其厚度减少４２．４０％。ＦＥＲＧ的ａ波和ｂ波振幅于光照后６小时及６天持续降低，至１４天仍无显著恢复。结论该模型可能是研究视网膜光化学损伤的较理想的动物模型。
Objective To observe the dynamic change of photochemical damage induced by green fluorescent lamp in rat retina. Methods Sprague-Dawley rats were exposed to (1900 ± 106.9) Lx green fluorescent light for 24 hours. Morphological changes of retina were observed before light, 6 hours, 6 days and 14 days after light exposure, respectively. FERG test. The results of the normal retinal light before the light, clear structure, internal and external arrangement of neat rules. After 6 hours of illumination, the outer retinal layer became thinner and its thickness decreased by 23.91%. The internal and external segments were disordered. The outer nuclear layer became thinner 6 days after light exposure, with a reduction of 46.60% in thickness. The 14-day outer nuclear layer thickened more than 6 days and the thickness decreased by 42.40%. The a-wave and b-wave amplitudes of FERG continued to decrease at 6 and 6 days after light exposure, with no significant recovery until 14 days. Conclusion This model may be a better animal model to study the photochemical damage of retina.