【摘 要】
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Purpose: To explore whether the antisense RNA ofMHC-Ⅱ gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB)cells.
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Purpose: To explore whether the antisense RNA ofMHC-Ⅱ gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB)cells.The authors transduced the antisense RNA ofMHC-Ⅱ gene into CD34 CB stem/progenitor cells.Methods: A retroviral vector encoding antisense RNA ofHLA-DR gene was constructed by molecular cloningtechnique and transducted into packaging cells line PA317by lipofectin. The high titer, helper-free, virus
Purpose: To explore whether the antisense RNA of MHC-II gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB) cells. The authors transduced the antisense RNA of MHC-II gene into CD34 CB stem / progenitor cells. Methods: A retroviral vector encoding antisense RNA of HLA-DR gene was constructed by molecular cloning technique and transducted into packaging cells line PA317by lipofectin. The high titer, helper-free, virus
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