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目的 了解狼疮肾炎 (LN)肾组织中NF κB表达并探讨其在LN中的作用。方法 以NF κB亚基 p6 5单抗为抗体 ,采用微波免疫组织化学法 (APAAP法 )染色 ,检测LN肾组织NF κB表达 ,并进一步分析其与LN活动指数、肾脏病理和功能损害的关系。结果 狼疮肾炎组织中NF κB表达较正常肾组织显著增高 ,尤以WHOⅣ型为甚。NF κB在肾小球和肾小管均有表达 ,但以肾小管表达更明显。LN肾组织NF κB阳性细胞数与肾组织活动指数、肾脏病理改变和肾功能损害显著相关。结论 NF κB可能参与了LN的发病机制 ,检测其在肾组织中的表达可作为反映狼疮肾组织活动病变和进行性肾损害的参考指标。
Objective To investigate the expression of NF κB in lupus nephritis (LN) and its role in LN. Methods NF - κB subunit p6 5 monoclonal antibody was used as an antibody and the expression of NF κB was detected by microwave immunohistochemical staining (APAAP). The relationship between NF κB and LN activity index, renal pathology and functional impairment was further analyzed. Results The expression of NF κB in lupus nephritis tissues was significantly higher than that in normal kidney tissues, especially in WHO Ⅳ type. NF κB is expressed in glomeruli and tubules, but more pronounced in tubules. The number of NF κB positive cells in LN was significantly correlated with renal activity index, renal pathological changes and renal dysfunction. Conclusion NF κB may be involved in the pathogenesis of LN, and its expression in renal tissue can be used as a reference index reflecting the pathological changes of renal lupus nephritis and progressive renal damage.