本文报导将葡萄糖氧化酶固定在改性硅胶上的最适宜条件及用此固相化酶测定葡萄糖的条件。同时拟定了模拟酶代替辣根过氧化物酶进行催化显色的反应条件。用所建立的分光光度法在固相化酶柱上测定了血清中葡萄糖的含量。与试剂盒测定结果对照，相关性较好，回收率为９５％～１０３％。酶柱历时两年多，重复测试５００次仍未失活。 This article reports the optimum conditions for the immobilization of glucose oxidase on modified silica gel and the conditions for the determination of glucose using this immobilized enzyme. At the same time, the reaction conditions of mimic enzyme instead of horseradish peroxidase were developed. With the established spectrophotometry in the immobilized enzyme column determination of serum glucose content. Compared with the kit test results, the correlation is good, the recovery rate is 95% ~ 103%. Enzyme column lasted more than two years, 500 repeated tests have not been inactivated.